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Culture, Genital

Vaginal, cervical, urethral

Test Codes

EPIC: LAB5491

Department

Microbiology

Instructions

• For prenatal screening, please see Group B Strep Screen and Chlamydia trachomatis and Neisseria gonorrhoeae Detection by Nucleic Acid Amplification.
• For biopsy specimens, please see Culture, Tissue.
• If Neisseria gonorrhoeae is suspected:
  1. The optimal detection method is Neisseria gonorrhoeae Detection by Nucleic Acid Amplification. Refer to the prior link for specimen collection and transport information.
  2. If drug resistance is of concern, or if performing a test of cure, collect a cervical swab (female) or urethral swab (male) and place in the ESwab transport system or Amies charcoal media (preferred). Please refer to Culture, GC. It is imperative that the specimen be received by the laboratory within 2 hours of collection if using the ESwab transport system or within 8 hours of collection if using Amies charcoal media for specimen transport.

Specimen Collection Criteria

Collect: Aseptically collect one of the following specimens:

• Cervical, urethral, vaginal, penile, seminal fluid, or labia swab specimen submitted in a transport system with Amies (e.g. ESwab or Culturette) or Charcoal Amies media.
• Seminal fluid in a sterile collection container.

Physician Office/Draw Specimen Preparation

Do NOT refrigerate specimen. Maintain at room temperature (20-26°C or 68-78.8°F) and transport to the Laboratory within 8 hours of collection.

Preparation for Courier Transport

Transport: Swab specimen in ESwab transport system or Amies charcoal medium, at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

• Specimens submitted on dry swabs or not in the listed transport media.
• Specimens delayed in transit (greater than 8 hours from the time of collection).

Inpatient Specimen Preparation

Immediately transport specimens to the Laboratory for processing.

In-Lab Processing

Do NOT refrigerate specimen. Maintain at room temperature (20-26°C or 68-78.8°F) prior to testing.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Room Temperature (20-26°C or 68-78.8°F): 4 days

Laboratory

Dearborn Microbiology Laboratory
Taylor, Trenton and Wayne sent to Dearborn Microbiology Laboratory for testing.

Royal Oak Microbiology Laboratory
Farmington Hills, Grosse Pointe, and Troy sent to Royal Oak Microbiology Laboratory for testing.

Performed

Sunday – Saturday, 24 hours a day.
Preliminary results available within 24 hours.

Reference Range

No pathogens recovered.

Test Methodology

Aerobic culture, identification, and susceptibility testing (if indicated).

Interpretation

The recovery of a bacteria from an appropriate, properly collected specimen may be indicative of an infectious disease process.

Clinical Utility

This test is used to recover and identify aerobic bacteria that may be involved in human infections.

Disease Reporting

Neisseria gonorrhoeae positive cultures are reportable to MDHHS per Michigan Law.

Reference

1. Vandamme, P.A.R. 2015. Specimen Collection, Transport, and Processing: Bacteriology. Jorgensen, J.H., Editor-in-Chief. Manual of Clinical Microbiology. 11^th edition. ASM Press. Washington, D.C., p. 228-272.
2. Linscott, A.J., 2016. Specimen Collection, Transport, and Acceptability. Clinical Microbiology Procedure Handbook. 4^th ed. Leber, A.L. (ed), ASM Press. Washington, D.C., Section 2.1.
3. Carson, J.A., 2016. Wound/Abscess and Soft Tissue Cultures. Clinical Microbiology Procedure Handbook, 4^th ed. Leber, A.L., (ed), ASM Press. Washington, D.C., Section 3.13.

CPT Codes

87070, 87088 (if indicated).
LOINC:  10352-3

Contacts

Last Updated

8/2/2022