Culture, Quantitative Bacterial, Tissue
Biopsy, Quantitative
Test Codes
EPIC: LAB5499
Department
Microbiology
Specimen Collection Criteria
Collect: Tissue specimen in a sterile collection container. A few drops of sterile non-bacteriostatic saline may be placed in the container to keep the specimen moist. (Minimum specimen size: 2 cm x 1 cm).
Physician Office/Draw Specimen Preparation
Do NOT refrigerate. Specimens must be stored at room temperature (20-26°C or 68-78.8°F). Courier Services must be contacted for a STAT pickup at 1-800-551-0488.
Preparation for Courier Transport
Transport: Tissue specimens at room temperature (20-26°C or 68-78.8°F).
Rejection Criteria
- Insufficient specimens (less than 2 cm X 1 cm).
- Specimens received on a swab.
- Specimens submitted in Formalin or in a container that ever contained Formalin.
- Frozen specimens.
Inpatient Specimen Preparation
Transport STAT to the Laboratory immediately after collection.
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 4 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Frozen (-20°C/-4°F or below): 1 month
Laboratory
Royal Oak Microbiology Laboratory
Dearborn, Farmington Hills, Grosse Pointe, Taylor, Trenton, Troy and Wayne sent to Royal Oak Microbiology Laboratory for testing.
Performed
Sunday – Saturday, 24 hours a day.
Preliminary results available within 24 hours.
Reference Range
Less than 100,000 (105) organisms per gram of tissue.
Test Methodology
Quantitative Culture.
Interpretation
Greater than 100,000 (105) organisms per gram of tissue is significant.
Clinical Utility
The presence of bacteria in tissue in significant amounts is one factor that has been associated with delayed healing and has been correlated with infection.
Reference
- York, M.K. 2010. Quantitative Cultures of Wound Tissues, Clinical Microbiology Procedures Handbook, 3rd edition updated. L.S. Garcia, Editor-in-Chief. ASM Press. Washington, D.C. page 3.13.2.
CPT Codes
87071 (Culture), 87205 (Gram Smear).
LOINC: 627-0
Contacts
Microbiology Laboratory – DBN
313-593-7921
Name: Microbiology Laboratory – DBN
Location:
Phone: 313-593-7921
Microbiology Laboratory – RO
248-551-8090
Name: Microbiology Laboratory – RO
Location:
Phone: 248-551-8090
Last Updated
4/5/2022
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