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Lab Test

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Glucose Phosphate Isomerase, Erythrocytes

Test Codes

MAYO: GPI, EPIC: LAB6039, SOFT: XGPI

Department

Send Outs

Specimen Collection Criteria

Collect: One Yellow-top ACD (Solution B) tube.
Also acceptable: Two Lavender-top EDTA tubes.

Send specimen for processing immediately after collection. 

Physician Office/Draw Specimen Preparation

Do not centrifuge or freeze. Maintain whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F), prior to transport.

Preparation for Courier Transport

Transport: 6.0 mL whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

  • Centrifuged specimens.
  • Frozen specimens.
  • Grossly hemolyzed specimens.
  • Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge or freeze. Maintain whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F), prior to transport.

Transport: 6.0 whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 20 days
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Medical Laboratories, Rochester, MN.

Performed

Monday – Friday.
Results available in 2-5 days.

Reference Range

By report.

Test Methodology

Kinetic Spectrophotometry.

Interpretation

By report.

Clinical Utility

This assay is used as a second order test in the evaluation of individuals with chronic hemolysis. Erythrocyte glucose phosphate isomerase (GPI) deficiency is a common cause of chronic hemolysis. GPI is an autosomal recessive trait. Hemolytic disease of the newborn is a common presenting manifestation of GPI deficiency.

CPT Codes

84087
LOINC:  44050-3

Contacts

Last Updated

7/7/2021

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