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MYD88 L265P Gene Mutation Analysis

MYD88 Gene Mutation, lymphoplasmacytic lymphoma, Waldenstrom macroglobulinemia, IgM, monoclonal gammopathy of undetermined significance, MYD, L265P, Waldenstrom, GMYDG

Test Codes

EPIC: LAB7107, SOFT: GMYDG

Specimen Collection Criteria

Collect: Blood OR Bone Marrow.

• Blood: 10 mL in Lavender-top EDTA tubes or Yellow-top ACD tubes. (Minimum: 5.0 mL)
• Bone Marrow: 1.0 mL in a Lavender-top EDTA tube. (Minimum: 0.5 mL)

Physician Office/Draw Specimen Preparation

Do not freeze specimens. Maintain specimen at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood or bone marrow at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

• Frozen specimens.
• Unlabeled tubes.
• Specimens collected in heparin (Green-top), clot tubes (Red-top), or SST (Gold-top) tubes.

In-Lab Processing

Do not freeze specimens. Maintain specimen refrigerated (2-8°C or 36-46°F) prior to testing.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Extracted DNA may be available for additional testing if clinically indicated. Contact the Molecular Pathology Laboratory for verification.

Laboratory

Royal Oak Clinical Molecular Pathology Laboratory

Performed

Once per week.
Results available in 7-10 days.

Test Methodology

Genomic DNA is isolated from peripheral blood or bone marrow aspirate. Allele-specific real-time PCR is used to detect the MYD88 L265P point mutation.

Interpretation

The test detects a point mutation in the MYD88 gene (MYD88 p.L265P). Results are reported as positive or negative.

Clinical Utility

• The MYD88 L265P point mutation ……
  • is detected in most patients with Waldenstrom macroglobulinemia / lymphoplasmacytic lymphoma and a significant proportion of patients with IgM monoclonal gammopathy of undetermined significance.
  • is infrequently detected in patients with chronic lymphocytic leukemia, gastric-associated lymphoid tissue lymphoma, marginal zone lymphoma and diffuse large B-cell lymphoma (activated B-cell type).
  • is not detected in patients with plasma cell myeloma, including IgM myeloma.

Guidelines for Cytogenetic/Molecular Testing of Hematopoietic Malignancy Updated

Reference

Negative (no mutation).

CPT Codes

81305

Contacts

Last Updated

8/27/2021