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Platelet Antigen Typing Panel

Platelet Antigen Typing (Level 1, 2, and 3), Platelet Typing

Test Codes

Blood Center of Wisconsin: 5600, EPIC: LAB6441, SOFT: XPLAG

Instructions

• The Platelet Antigen Typing Panel will only be performed after a normal result for Platelet Glycoprotein Polymorphism Analysis (PL A1/A2).
• If a patient has received a whole blood or platelet transfusion, wait 24 hours before drawing sample.  Please indicate on the requisition when the patient received the most recent transfusion.
• Please provide patient's platelet count on requisition, if known.

Specimen Collection Criteria

Collect:

• Whole Blood: Two Lavender-top EDTA tubes.
• Fetal Specimen: 15 mL of amniotic fluid, 5x10⁶ amniocytes cultured fresh, or cord blood.

Physician Office/Draw Specimen Preparation

Maintain whole blood or fetal specimens at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood or fetal specimens, at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Maintain whole blood or fetal specimens at room temperature (20-26°C or 68-78.8°F) prior to transport.

Transport: Whole blood or fetal specimens, at room temperature (20-26°C or 68-78.8°F).

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 96 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Blood Center of Wisconsin, Milwaukee, WI.

Performed

Varies.
Results are available in 8-9 days.

Reference Range

By report.

Test Methodology

Taqman Polymerase Chain Reaction (PCR), Allele Specific Probes Genotyping.

Interpretation

Platelet Antigen Typing (Level 1,2, and 3).

• Level 1 must be done before Level 2 or 3 can be ordered.
• Level 1 includes: Pl^A1/A2, Br^a/b, Bak^a antigens.
• Level 2 includes: Bak^b, Ko^a/b, Pen^a/b antigens.
• Level 3 includes: Ca^a/b antigens.

Clinical Utility

If this assay is ordered alone the clinical significance is up to the doctor. This assay is usually ordered as a panel with other tests to rule out post-transfusion purpura or neonatal alloimmune thrombocytopenia.

CPT Codes

81105, 81106, 81107, 81108, 81109, 81110, 81111, 81112.

Contacts

Last Updated

4/10/2021