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PML/RARA Quantitative by PCR

Acute promyelocytic leukemia (APL), acute myeloid leukemia (AML)

Test Codes

MAYO: PMLR, EPIC: LAB6801, SOFT: XPML

Department

Send Outs

Instructions

Due to specimen stability, patients can be drawn Monday – Thursday only. For questions, call the Laboratory at 800-551-0488. The following information is required:

1. Pertinent clinical history-please include if this is to be considered a diagnostic specimen for the purposes of future monitoring.
2. Clinical or morphologic suspicion.
3. Date and time of collection.
4. Specimen source.

Specimen Collection Criteria

Collect (preferred specimen): Blood or bone marrow in one Lavender-top EDTA tube. (Minimum: 4.0 mL blood, 2.0 mL bone marrow).
Also acceptable: Blood or bone marrow in one Yellow-top ACD tube. (Minimum: 4.0 mL blood, 2.0 mL bone marrow).

Invert several times to mix blood or bone marrow.

Physician Office/Draw Specimen Preparation

Do not centrifuge. Maintain specimen in original collection tube, refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: 10.0 mL blood or 4.0 mL bone marrow, refrigerated (2-8°C or 36-46°F) in original collection tube. (Minimum: 4.0 mL blood, 2.0 mL bone marrow).

Rejection Criteria

• Grossly hemolyzed specimens.
• Specimens that do NOT arrive at Mayo Medical Laboratories within 5 days.
• Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge. Maintain specimen in original collection tube, refrigerated (2-8°C or 36-46°F) prior to transport.

Transport: 10.0 mL blood or 4.0 mL bone marrow, refrigerated (2-8°C or 36-46°F) in original collection tube. (Minimum: 4.0 mL blood, 2.0 mL bone marrow).

Storage

Specimen Stability for Testing:

Room temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 120 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Send to Mayo Medical Laboratories, Rochester, MN.

Performed

Monday – Friday.
Results available in 5-9 days.

Reference Range

By report.

Test Methodology

Quantitative, Real-Time Polymerase Chain Reaction (PCR).

Interpretation

By report.

CPT Codes

81315

Contacts

Last Updated

4/10/2021