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Adenovirus, Quantitative PCR

Test Codes

ARUP: 2007192, EPIC: LAB7582, Beaker: XADVQ

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Lavender-top EDTA tube.
Also acceptable: One gold-top SST tube.

Physician Office/Draw Specimen Preparation

Whole blood: Transport 1mL whole blood, refrigerated (2-8°C or 36-46°F).

Serum or plasma: Centrifuge to separate serum or plasma from cells. Transfer 1mL serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)

Rejection Criteria

• Frozen whole blood.
• Specimens not collected and processed as indicated.

In-Lab Processing

Whole blood: Transport 1mL whole blood, refrigerated (2-8°C or 36-46°F).

Serum or plasma: Centrifuge to separate serum or plasma from cells. Transfer 1mL serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 365 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Sunday – Saturday
Results available in 2-5 days.

Reference Range

By report.

Test Methodology

Quantitative Real-Time Polymerase Chain Reaction.

Interpretation

The quantitative range of this assay is 3.0- 7.0 log copies/mL (1,000-10,000,000 copies/mL).

Clinical Utility

Detect and quantify adenovirus groups A-F. Diagnose adenovirus in patients. Monitor disease and/or response to therapy. A negative result (less than 3.0 log copies/mL or less than 1,000 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or Adenovirus DNA concentrations below the level of detection of the assay.  Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.

CPT Codes

87799
LOINC:  31208-2, 49340-3, 64038-3, 39528-5

Contacts

Last Updated

7/16/2021