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HLA Antibody Screen

HLA Antibody Screening

Test Codes

EPIC: LAB6420, SOFT: HLABS

Department

HLA

Specimen Collection Criteria

Collect: One Gold-top SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transport to the Laboratory within 48 hours of collection.

Preparation for Courier Transport

Transport: Centrifuged SST tube, at room temperature (20-25°C or 68-77°F).

Rejection Criteria

• Unlabeled specimens.
• Specimens drawn in Sodium Heparin tubes.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube and refrigerate (2-8°C or 36-46°F) prior to testing.

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Room Temperature (20-26°C or 68-78.8°F): Indefinitely

Laboratory

Royal Oak HLA Laboratory

Performed

Monday – Friday, 7:00 am – 5:00 pm.
Results available within 7 days.

Reference Range

Positive: Normalized Fluorescence Intensity greater than 1000.

Test Methodology

Luminex Multiplex Bead Assay.

Clinical Utility

Anti-HLA antibody identification for pre- and post-transplant monitoring.

CPT Codes

86832, 86833.

Contacts

Last Updated

8/4/2021