Scleroderma (SCL-70) Antibody (IgG)
Scleroderma antibodies, Scleroderma Antibody (SCL-70)
Test Codes
Antrim #30080, EPIC: LAB6988, SOFT: SCL7A
Department
Special Chemistry
Specimen Collection Criteria
Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)
Physician Office/Draw Specimen Preparation
Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)
Preparation for Courier Transport
Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)
Rejection Criteria
Plasma specimens.
Severely hemolyzed, lipemic or icteric specimens.
In-Lab Processing
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)
Storage
Specimen Stability for Testing:
Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable
Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days
Laboratory
Royal Oak Special Testing Laboratory
Performed
Monday – Friday.
Results available within 2 business days.
Reference Range
Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.
Test Methodology
Multiplex Bead Immunoassay.
Interpretation
SCL-70 antibody is seen in 20-60% of scleroderma cases. The presence of SCL-70 antibodies is considered diagnostic for scleroderma if it is the only ENA antibody present. Scl-70 is also seen in approximately 25% of progressive systemic sclerosis (PSS) cases.
Clinical Utility
SCL-70 antibody assay aids in the diagnosis of scleroderma and progressive systemic sclerosis (PSS).
CPT Codes
86235
Contacts
Special Chemistry Laboratory – RO
248-551-8044
Name: Special Chemistry Laboratory – RO
Location:
Phone: 248-551-8044
Last Updated
6/20/2022
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